t7 mscript tm Search Results


smai  (New England Biolabs)
96
New England Biolabs smai
Smai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smai/product/New England Biolabs
Average 96 stars, based on 1 article reviews
smai - by Bioz Stars, 2026-03
96/100 stars
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t7 mscript(tm) standard mrna production system  (Cellscript Inc)
90
Cellscript Inc t7 mscript(tm) standard mrna production system
( A ) Upstream transcription regulators inferred for each iEGA time-point (INGENUITY). ( B ) Hoffman micrographs of embryos produced by in vitro fertilization (IVF) and incubated in c-Myc inhibitor, 10058-F4 (0, 1 or 2 μM) after six (upper row) or 24 h. Scale bar, 100 μm. ( C ) Quantification of developmental rates (percentage of embryos surviving IVF) of embryos of ( B ) for n =3 independent biological replicates. 2-cell, two-cell embryo (24 h after IVF); 4-cell, four-cell embryo (∼48 h); eB, expanded blastocyst (∼96 h). ( D ) Histogram plots of transcript levels determined by qPCR using intron-flanking primers for random-primed cDNA derived from metaphase II (mII) oocytes (open bars) and embryos 6 h after in vitro fertilization. Intron-exon primer pairs gave products for genomic DNA but not cDNA. ( E ) qPCR analysis with intra-exonic primers and primers flanking exon-exon junctions. ( F ) Spliceosome component and guanylyltransferase ( Rngtt ) transcript levels determined by qPCR in germinal vesicle (GV) oocytes, mII oocytes (0) and one-cell embryos at the times shown (h) after sperm injection. ( G ) Injection of mCherry cRNA (mCh, top left: orf, mCherry open reading frame; c, cytoplasmic polyadenylation element; t, <t>mRNA</t> cleavage/polyadenylation signal). Fluorescence intensity quantification (lower left) at the times shown after injection of mCherry cRNA (0.6 ng/μl) polyadenylated in vitro (pA + ) or not (pA - ). Fluorescence micrographs show representative oocytes with corresponding bright field images (insets, upper left) and a non-injected control (neg, inset upper right). Bars, 100 μm. Values in ( C - G ) are ± s.e.m. Unpaired t -tests show p -values <0.05.
T7 Mscript(Tm) Standard Mrna Production System, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7 mscript(tm) standard mrna production system/product/Cellscript Inc
Average 90 stars, based on 1 article reviews
t7 mscript(tm) standard mrna production system - by Bioz Stars, 2026-03
90/100 stars
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t7 mscript tm kit  (Cellscript Inc)
90
Cellscript Inc t7 mscript tm kit
( A ) Upstream transcription regulators inferred for each iEGA time-point (INGENUITY). ( B ) Hoffman micrographs of embryos produced by in vitro fertilization (IVF) and incubated in c-Myc inhibitor, 10058-F4 (0, 1 or 2 μM) after six (upper row) or 24 h. Scale bar, 100 μm. ( C ) Quantification of developmental rates (percentage of embryos surviving IVF) of embryos of ( B ) for n =3 independent biological replicates. 2-cell, two-cell embryo (24 h after IVF); 4-cell, four-cell embryo (∼48 h); eB, expanded blastocyst (∼96 h). ( D ) Histogram plots of transcript levels determined by qPCR using intron-flanking primers for random-primed cDNA derived from metaphase II (mII) oocytes (open bars) and embryos 6 h after in vitro fertilization. Intron-exon primer pairs gave products for genomic DNA but not cDNA. ( E ) qPCR analysis with intra-exonic primers and primers flanking exon-exon junctions. ( F ) Spliceosome component and guanylyltransferase ( Rngtt ) transcript levels determined by qPCR in germinal vesicle (GV) oocytes, mII oocytes (0) and one-cell embryos at the times shown (h) after sperm injection. ( G ) Injection of mCherry cRNA (mCh, top left: orf, mCherry open reading frame; c, cytoplasmic polyadenylation element; t, <t>mRNA</t> cleavage/polyadenylation signal). Fluorescence intensity quantification (lower left) at the times shown after injection of mCherry cRNA (0.6 ng/μl) polyadenylated in vitro (pA + ) or not (pA - ). Fluorescence micrographs show representative oocytes with corresponding bright field images (insets, upper left) and a non-injected control (neg, inset upper right). Bars, 100 μm. Values in ( C - G ) are ± s.e.m. Unpaired t -tests show p -values <0.05.
T7 Mscript Tm Kit, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7 mscript tm kit/product/Cellscript Inc
Average 90 stars, based on 1 article reviews
t7 mscript tm kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
t7 mscript tm  (Cellscript Inc)
90
Cellscript Inc t7 mscript tm
( A ) Upstream transcription regulators inferred for each iEGA time-point (INGENUITY). ( B ) Hoffman micrographs of embryos produced by in vitro fertilization (IVF) and incubated in c-Myc inhibitor, 10058-F4 (0, 1 or 2 μM) after six (upper row) or 24 h. Scale bar, 100 μm. ( C ) Quantification of developmental rates (percentage of embryos surviving IVF) of embryos of ( B ) for n =3 independent biological replicates. 2-cell, two-cell embryo (24 h after IVF); 4-cell, four-cell embryo (∼48 h); eB, expanded blastocyst (∼96 h). ( D ) Histogram plots of transcript levels determined by qPCR using intron-flanking primers for random-primed cDNA derived from metaphase II (mII) oocytes (open bars) and embryos 6 h after in vitro fertilization. Intron-exon primer pairs gave products for genomic DNA but not cDNA. ( E ) qPCR analysis with intra-exonic primers and primers flanking exon-exon junctions. ( F ) Spliceosome component and guanylyltransferase ( Rngtt ) transcript levels determined by qPCR in germinal vesicle (GV) oocytes, mII oocytes (0) and one-cell embryos at the times shown (h) after sperm injection. ( G ) Injection of mCherry cRNA (mCh, top left: orf, mCherry open reading frame; c, cytoplasmic polyadenylation element; t, <t>mRNA</t> cleavage/polyadenylation signal). Fluorescence intensity quantification (lower left) at the times shown after injection of mCherry cRNA (0.6 ng/μl) polyadenylated in vitro (pA + ) or not (pA - ). Fluorescence micrographs show representative oocytes with corresponding bright field images (insets, upper left) and a non-injected control (neg, inset upper right). Bars, 100 μm. Values in ( C - G ) are ± s.e.m. Unpaired t -tests show p -values <0.05.
T7 Mscript Tm, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7 mscript tm/product/Cellscript Inc
Average 90 stars, based on 1 article reviews
t7 mscript tm - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Upstream transcription regulators inferred for each iEGA time-point (INGENUITY). ( B ) Hoffman micrographs of embryos produced by in vitro fertilization (IVF) and incubated in c-Myc inhibitor, 10058-F4 (0, 1 or 2 μM) after six (upper row) or 24 h. Scale bar, 100 μm. ( C ) Quantification of developmental rates (percentage of embryos surviving IVF) of embryos of ( B ) for n =3 independent biological replicates. 2-cell, two-cell embryo (24 h after IVF); 4-cell, four-cell embryo (∼48 h); eB, expanded blastocyst (∼96 h). ( D ) Histogram plots of transcript levels determined by qPCR using intron-flanking primers for random-primed cDNA derived from metaphase II (mII) oocytes (open bars) and embryos 6 h after in vitro fertilization. Intron-exon primer pairs gave products for genomic DNA but not cDNA. ( E ) qPCR analysis with intra-exonic primers and primers flanking exon-exon junctions. ( F ) Spliceosome component and guanylyltransferase ( Rngtt ) transcript levels determined by qPCR in germinal vesicle (GV) oocytes, mII oocytes (0) and one-cell embryos at the times shown (h) after sperm injection. ( G ) Injection of mCherry cRNA (mCh, top left: orf, mCherry open reading frame; c, cytoplasmic polyadenylation element; t, mRNA cleavage/polyadenylation signal). Fluorescence intensity quantification (lower left) at the times shown after injection of mCherry cRNA (0.6 ng/μl) polyadenylated in vitro (pA + ) or not (pA - ). Fluorescence micrographs show representative oocytes with corresponding bright field images (insets, upper left) and a non-injected control (neg, inset upper right). Bars, 100 μm. Values in ( C - G ) are ± s.e.m. Unpaired t -tests show p -values <0.05.

Journal: bioRxiv

Article Title: Mouse fertilization triggers a conserved transcription program in one-cell embryos

doi: 10.1101/2020.09.15.298018

Figure Lengend Snippet: ( A ) Upstream transcription regulators inferred for each iEGA time-point (INGENUITY). ( B ) Hoffman micrographs of embryos produced by in vitro fertilization (IVF) and incubated in c-Myc inhibitor, 10058-F4 (0, 1 or 2 μM) after six (upper row) or 24 h. Scale bar, 100 μm. ( C ) Quantification of developmental rates (percentage of embryos surviving IVF) of embryos of ( B ) for n =3 independent biological replicates. 2-cell, two-cell embryo (24 h after IVF); 4-cell, four-cell embryo (∼48 h); eB, expanded blastocyst (∼96 h). ( D ) Histogram plots of transcript levels determined by qPCR using intron-flanking primers for random-primed cDNA derived from metaphase II (mII) oocytes (open bars) and embryos 6 h after in vitro fertilization. Intron-exon primer pairs gave products for genomic DNA but not cDNA. ( E ) qPCR analysis with intra-exonic primers and primers flanking exon-exon junctions. ( F ) Spliceosome component and guanylyltransferase ( Rngtt ) transcript levels determined by qPCR in germinal vesicle (GV) oocytes, mII oocytes (0) and one-cell embryos at the times shown (h) after sperm injection. ( G ) Injection of mCherry cRNA (mCh, top left: orf, mCherry open reading frame; c, cytoplasmic polyadenylation element; t, mRNA cleavage/polyadenylation signal). Fluorescence intensity quantification (lower left) at the times shown after injection of mCherry cRNA (0.6 ng/μl) polyadenylated in vitro (pA + ) or not (pA - ). Fluorescence micrographs show representative oocytes with corresponding bright field images (insets, upper left) and a non-injected control (neg, inset upper right). Bars, 100 μm. Values in ( C - G ) are ± s.e.m. Unpaired t -tests show p -values <0.05.

Article Snippet: 5’-capped cRNA was synthesized in vitro from linearized plasmid template DNA in a T7 mScript(tm) Standard mRNA Production System (Cellscript, USA) according to the recommendations of the manufacturer, as previously described , , .

Techniques: Produced, In Vitro, Incubation, Random Primed, Derivative Assay, Injection, Fluorescence, Control